Question: (Closed) Troubles in de novo assembly of an insect genome
gravatar for aida.shahraki
8 months ago by
aida.shahraki10 wrote:

I need to assemble an insect genome. The genome size is around 500Mb. I have performed genome sequencing in illumina platform, 150bp paired-end reads. I have trimmed forward and reverse reads and each of this files is around 45Gb. I have tried to assemble this dataset using SOAPdenovo and Abyss, but the process has killed for each try. Actually, I am running Abyss on a system with fairly good RAM capacity (128GB) but still, it is killed. I have tried at 64 and 96 Kmers. I do really need to assemble dataset as soon as it is possible, so I would be so grateful if anyone help me how can I tackle the problem.

abyss soap assembly • 286 views
ADD COMMENTlink written 8 months ago by aida.shahraki10

Hello aida.shahraki!

We believe that this post does not fit the main topic of this site.

This forum is for questions about using Galaxy For general bioinformatics help, please try

For this reason we have closed your question. This allows us to keep the site focused on the topics that the community can help with.

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ADD REPLYlink written 8 months ago by Jennifer Hillman Jackson25k
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