Question: genome de novo assembly
gravatar for aida.shahraki
5 days ago by
aida.shahraki0 wrote:

Hello, I have a paired-end 150 bp genome sequencing data with a different end quality.According to the fastQC analysis, the 10 last nucleotides of one read should be trimmed, while for the other one 30 nucleotides need to be trimmed. Can I trim different numbers of nucleotides from each read and then assemble them? Or, as it paired-end it should be same? Regards, Aida

ADD COMMENTlink modified 4 days ago by Jennifer Hillman Jackson24k • written 5 days ago by aida.shahraki0
gravatar for Jennifer Hillman Jackson
4 days ago by
United States
Jennifer Hillman Jackson24k wrote:


Please see the Galaxy Assembly tutorials here for an overview of how to use the tools (with more details linked from the underlying tool's manuals):

In short, some tools expect a consistent insert size/sequence lengths and others are more flexible.

All Galaxy tutorials:

Thanks! Jen, Galaxy team

ADD COMMENTlink written 4 days ago by Jennifer Hillman Jackson24k
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