Question: genome de novo assembly
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gravatar for aida.shahraki
3 months ago by
aida.shahraki10 wrote:

Hello, I have a paired-end 150 bp genome sequencing data with a different end quality.According to the fastQC analysis, the 10 last nucleotides of one read should be trimmed, while for the other one 30 nucleotides need to be trimmed. Can I trim different numbers of nucleotides from each read and then assemble them? Or, as it paired-end it should be same? Regards, Aida

ADD COMMENTlink modified 3 months ago by Jennifer Hillman Jackson25k • written 3 months ago by aida.shahraki10
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gravatar for Jennifer Hillman Jackson
3 months ago by
United States
Jennifer Hillman Jackson25k wrote:

Hello,

Please see the Galaxy Assembly tutorials here for an overview of how to use the tools (with more details linked from the underlying tool's manuals): http://galaxyproject.github.io/training-material/topics/assembly/

In short, some tools expect a consistent insert size/sequence lengths and others are more flexible.

All Galaxy tutorials: https://galaxyproject.org/learn/

Thanks! Jen, Galaxy team

ADD COMMENTlink written 3 months ago by Jennifer Hillman Jackson25k
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