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• T to C mutations in PAR-CLIP
  HI: I am looking for a way to quantify T to C changes on my PAR-CLIP data. Tools such as Naive V...
• Removing a single base from 3' end of the reads using "Clip" option
  Hello, I need to remove a single base i.e. C from the 3' end of all of my reads. I tried doing i...
• Setting clipping sequence as workflow parameter when running FASTX Clipper from BioBlend API
  I am using the BioBlend API to run a workflow on Galaxy, and I am having trouble setting the para...
• PAR-CLIP data analysis
  Hi: Do you have a suggested pipeline for the analysis of PAR-CLIP data (cross-linking and immuno...
• Clip Adapter Sequences
  Hi We're a bit confused about exactly how the adaptor clipping tool works. Is there some documen...
• How do i remove multiple adapter sequences from my RNAseq reads?
  Hi there, I want to remove the universal adapters as well as the index adapters in each data fil...
• Primer Contamination, Miranalyzer
  Hi Galaxy, Ive got 2 problems for you; 1) Ive got microRNA Illumina NGS data that I want to ana...
• Need help with "Clip" tool
  hi, i have a question about this tool i heard that galaxy has 'trimmomatic' tool. But i can't f...
• ParClip analysis Question please give me information very detail.
  HI ! I would like analysis Par Clip Data set in NCBI GEO. so i read some paper that conducted Par...
• HISAT2 soft clipping
  When using default options HISAT2 performs soft clipping. Under scoring options soft clipping is...
• Difficulty with aaChanges
  Hi! We have a series of mutations that are referenced to HG19. We would like to know how these m...
• Fastq-Mcf
  Hi all, Does someone know fastq-MCF? We are relatively newbies in analysing RNAseq results, and w...
• Aachange And Sift Tools
  Hello, I have some sequencing results I want to blast in order to identify what are known SNPs a...
• Output of FLASH can't be read by other tools?
  Hi, I have used FLASH to assemble some reads. They are still in FASTq format. However I can't ge...
• Can't start Galaxy server
  I can't start up my Galaxy server, I tried running on both dev and master branches. I also tried ...