Question: How to convert .txt files into fastq file format
0
gravatar for mayurdoke
18 months ago by
mayurdoke • 10
mayurdoke • 10 wrote:

Hii all,

I am trying to do the analysis of Chip seq data. I have six .txt format files and each file has around 6gb data. I have successfully uploaded the file and ran fastqc analysis. Then I tried to use tool trimometric but I could not upload any file. What is the reason behind that? Do i have to convert my .txt format file into fasta format? how to do that? Please help me.

chip-seq • 1.8k views
ADD COMMENT • link • modified 18 months ago • written 18 months ago by mayurdoke • 10

Related question: https://biostar.usegalaxy.org/p/23362

ADD REPLY • link written 18 months ago by Jennifer Hillman Jackson ♦ 25k
1
gravatar for Devon Ryan
18 months ago by
Devon Ryan • 1.9k
Germany
Devon Ryan • 1.9k wrote:

It's likely that you just need to change the datatype attribute in the history item. Can you post the first 5-10 lines of one of the files though so we can be sure?

ADD COMMENT • link written 18 months ago by Devon Ryan • 1.9k
0
gravatar for mayurdoke
18 months ago by
mayurdoke • 10
mayurdoke • 10 wrote:

Thank you for reply.

Please find below the lines of data files.

@D00459:134:CA3WWANXX:8:2209:1149:1972 2:N:0:ATTCAGAA+ATAGAGGC AAAAAAAAAAAAAAAGGGCTGAAAAAACGGCTGGCCCAGCCAACGGTTGGCGTAACAAACGGCTGGCCCAGCCAACGGCCGGCCCGGAAAACGGCCGGCCCCACAAACGGCTGGCCCCCACAAAC + BBBBFFFFFFFFFFB/

ADD COMMENT • link written 18 months ago by mayurdoke • 10

Already fastq then, just change the datatype.

ADD REPLY • link written 18 months ago by Devon Ryan • 1.9k
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