Question: Combine multiple files in Galaxy
0
gravatar for ando.kelli
2.4 years ago by
ando.kelli • 0
ando.kelli • 0 wrote:

Hi all,

I have over 300 fasta files that I need to combine into a single fine.

I don't have command line experience, so want to do this in Galaxy.

I have tried using the concatenate datasets tool under text manipulation, however this won't work with so many files. This tool only seems useful for combining a few files at a time into one.

I thought this would be relatively straight forward. However, it's doing my head in a bit, and any advice would be greatly appreciated.

ADD COMMENT • link • modified 2.4 years ago by Jennifer Hillman Jackson ♦ 25k • written 2.4 years ago by ando.kelli • 0

Are you using a usegalaxy.org? There is a different cat tool that will help you!

ADD REPLY • link written 2.4 years ago by Bjoern Gruening ♦ 5.1k

Hi Bjoern,

No I'm using an Australian site (University of Queensland).

*ended up watching some Linux tutorials on YouTube and managed to use 'cat' to combine the files.

A few years back I was using the main usegalaxy.org, and at the time it had the BLAST2GO tool which I now need. I'll need to find another way to use that tool now.

Thanks for your help!

ADD REPLY • link written 2.4 years ago by ando.kelli • 0

Try to contact your Galaxy admins and ask them to install the tools. Otherwise you can use two Galaxy instances :)

ADD REPLY • link written 2.4 years ago by Bjoern Gruening ♦ 5.1k

Yeah I had the same thought. I couldn't easily find the tool in another public instance, and realised that I'll probably keep running into the same problem over and over.

I ended up biting the bullet and installing Galaxy and then several tools. I also had someone who's fluent in Linux sit with me and install a bunch of things to use directly through the command line. Such a big learning curve but will be worth it.

Thanks for your help!

ADD REPLY • link written 2.3 years ago by ando.kelli • 0
0
gravatar for Jennifer Hillman Jackson
2.4 years ago by
United States
Jennifer Hillman Jackson ♦ 25k wrote:

Hello,

Galaxy is probably not the best choice for this manipulation. The Concatenate tool was not designed to process 300+ datasets except when used through the API. Using "cat" on the command line is simpler/quicker than writing an API script unless this is something that you need/expect to do repeatedly, with a reproducible history, and plan to use other Galaxy tools downstream.

Perhaps someone in your work/lab group can help you?

Jen, Galaxy team

ADD COMMENT • link written 2.4 years ago by Jennifer Hillman Jackson ♦ 25k

Hi Jen,

Thanks for your help. I ended up doing some YouTube tutorials and did it using Linux.

Kel

ADD REPLY • link written 2.4 years ago by ando.kelli • 0
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