Question: Can I run the megablast against my own database?
0
gravatar for li.4001
2.9 years ago by
li.4001 • 0
United States
li.4001 • 0 wrote:

Hi,

I'm trying to fish out antibiotic resistant genes from a hiseq data generated from human stool. 

I have the antibiotic resistant gene database, how can I do it on Galaxy? I found that megablast only provide four default databases, how can I use my own?

Or anyone can recommend me some other tools to finish the job?

Thank you!

galaxy • 688 views
ADD COMMENT • link • modified 2.9 years ago by Jennifer Hillman Jackson ♦ 25k • written 2.9 years ago by li.4001 • 0
0
gravatar for Jennifer Hillman Jackson
2.9 years ago by
United States
Jennifer Hillman Jackson ♦ 25k wrote:

Hello,

Megablast at http://usegalaxy.org is indexed for specific databases. To use others, install the tool on a local or cloud Galaxy along with the indexes. 

https://wiki.galaxyproject.org/BigPicture/Choices

http://usegalaxy.org/toolshed

Best, Jen, Galaxy team

ADD COMMENT • link written 2.9 years ago by Jennifer Hillman Jackson ♦ 25k
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