Question: History clearance for new run
0
gravatar for apereira
3.0 years ago by
apereira • 20
United States
apereira • 20 wrote:

Hello,

I am trying to get rid of old history to load datasets for new run. Unfortunately, eventhough I did delete the history, it didn't clear up the space. Please let me know what needs to be done to make space.

 

thanks

 

galaxy • 832 views
ADD COMMENT • link • modified 2.9 years ago • written 3.0 years ago by apereira • 20

If I clear up some files in the history, it doesn't open up space. Do I need to  make a new history after I download  the important files for further run.

julie

ADD REPLY • link written 3.0 years ago by apereira • 20
0
gravatar for Jennifer Hillman Jackson
3.0 years ago by
United States
Jennifer Hillman Jackson ♦ 25k wrote:

Hello,

The data needs to be permanently deleted (purged), not just deleted. Here is how to find all of your data and do that: Learn/ManagingDatasets#Delete_vs_Delete_Permanently

Thanks, Jen, Galaxy team

ADD COMMENT • link written 3.0 years ago by Jennifer Hillman Jackson ♦ 25k

If I clear up some files in the history, it doesn't open up space. Do I need to  make a new history after I download  the important files for further run.

ADD REPLY • link written 3.0 years ago by apereira • 20

If you are working at http://usegalaxy.org, the space should just free up after permanently deleting. I just recalculated disk space on the Main server and it was unchanged. My guess is that the data is only deleted, not permanently deleted. Double check that and I will also go in and take a look to confirm that is the issue.

ADD REPLY • link modified 3.0 years ago • written 3.0 years ago by Jennifer Hillman Jackson ♦ 25k

Hello again, I can see both deleted and perm deleted datasets in your histories. To permanently delete all that are only deleted, use the history menu option "Purge Deleted Datasets".

ADD REPLY • link written 3.0 years ago by Jennifer Hillman Jackson ♦ 25k
1

Thanks!!Jennifer. It did clear up the space.

ADD REPLY • link written 3.0 years ago by apereira • 20

Great! Glad that worked. Jen

ADD REPLY • link written 2.9 years ago by Jennifer Hillman Jackson ♦ 25k
0
gravatar for apereira
2.9 years ago by
apereira • 20
United States
apereira • 20 wrote:

Hi  Jen,

Is there any tool in galaxy to --prefilter multi read hits in Galaxy TopHat?

 

julie

ADD COMMENT • link written 2.9 years ago by apereira • 20

Try SAMtools after alignment: Filter SAM or BAM, output SAM or BAM. 

Option on form: Filter on bitwise flag (choose Yes)

Primary alignments can be preserved or non-primary eliminated. Ties will still be present, but should be low in count and cannot really be teased apart unless done arbitrarily. In most cases I can think of, you'll want to retain these ties, as all are equally valid.

In Tophat, under advanced options: Maximum number of alignments to be allowed can be set to 1, but the read retained for ties will be arbitrarily chosen. Still, it is a choice.

Next time for a new question, please start up a new post.

Thanks, jen

ADD REPLY • link modified 2.9 years ago • written 2.9 years ago by Jennifer Hillman Jackson ♦ 25k
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