Question: How can I merge two single-end mapping data
0
gravatar for zhhxu9
4.2 years ago by
zhhxu9 • 0
Canada
zhhxu9 • 0 wrote:

Hi,

I am analysing my ChIP-Seq data which generated by paired-end sequencing. But due to some experimental issues. It is better to mapping both the R1 and R2 data in single-end way separately.

So I would like to know how can I combine two SAM or BAM files into one file and how can I get the mapping statistics (eg. mapped and unmapped percentage, unique and multiple mapped percentage...) from the combined file.

Thanks a lot.

Zhenhua

ADD COMMENT • link • modified 4.2 years ago by Bjoern Gruening ♦ 5.1k • written 4.2 years ago by zhhxu9 • 0
1
gravatar for Bjoern Gruening
4.2 years ago by
Bjoern Gruening ♦ 5.1k
Germany
Bjoern Gruening ♦ 5.1k wrote:

I may be completely wrong, but I would say that you can simply put both files together. To do this you can use the tool "Concatenate datasets tail-to-head".

Cheers,

Bjoern

ADD COMMENT • link written 4.2 years ago by Bjoern Gruening ♦ 5.1k

Thanks Bjoern, I will take a shot.

ADD REPLY • link written 4.2 years ago by zhhxu9 • 0
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