Question: Help! I'm using trimmomatic in Galaxy, but it doesn't recognize a part of input dataset.
gravatar for redgreen
17 months ago by
redgreen10 wrote:

I'm using Trimmomatic to trimming paired end reads. When I set "Single-end or paired-end reads?" to Paired-end (two separate input files), it recognizes files only for Input FASTQ file (R2/second of pair). Therefor, I can choose any fastq files for R2/second pair, on the other hand I could not choose no files for R1/first pair. How do I do to fix the problem??

rna-seq software error • 626 views
ADD COMMENTlink modified 16 months ago by Jennifer Hillman Jackson25k • written 17 months ago by redgreen10

My fastq files are paired end by they were already combined into one. Under trimmomatic, how do I input the fastq files?

ADD REPLYlink written 16 months ago by serrano.gus0
gravatar for Jennifer Hillman Jackson
16 months ago by
United States
Jennifer Hillman Jackson25k wrote:


The dataset "datatype" probably needs to be both confirmed to the in fastqsanger format then have that assigned.


Thanks, Jen, Galaxy team

ADD COMMENTlink written 16 months ago by Jennifer Hillman Jackson25k
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