For "percentage match", there are two interpretations.
1) what percent of your total data matches a particular taxonomic
2) what percent (coverage/identity) of a query sequence matches a
"hit", leading to a taxonomic assignment
The other tools in the group "Metagenomic analyses" all accept "Fetch
taxonomic representation" output as input, and produce various
graphical, and statistical information. Please give these tools a try
On these tool forms, the "Fetch taxonomic representation" tool is
referred to as "Taxonomy manipulation->Fetch Taxonomic Ranks", as I
noted in my prior email. This is a legacy naming related to the prior
publication, and we apologize if this still caused confusion. This
should probably be updated, I will bring it up with the team.
I sent instructions to Scott Tighe this morning with one example of
to use individual tools to select, sort, group, and filter data.
While the details for your analysis may differ, the basic tool set
probably be the same for your project. Filtering data by alignment
quality prior to "Fetch Taxonomic Representation" was also part of the
Metagenomics example in the publication we shared. The idea is to
with the parsed BLAST output, generate statistics, filter and group
based on those results, then go forward with Taxonomic assignments.
There are no automated tools for this process, in a single step.
Hopefully this helps to clear up the tool set,