Question: How can I merge two single-end mapping data
0
gravatar for zhhxu9
3.9 years ago by
zhhxu90
Canada
zhhxu90 wrote:

Hi,

I am analysing my ChIP-Seq data which generated by paired-end sequencing. But due to some experimental issues. It is better to mapping both the R1 and R2 data in single-end way separately.

So I would like to know how can I combine two SAM or BAM files into one file and how can I get the mapping statistics (eg. mapped and unmapped percentage, unique and multiple mapped percentage...) from the combined file.

Thanks a lot.

Zhenhua

ADD COMMENTlink modified 3.9 years ago by Bjoern Gruening5.1k • written 3.9 years ago by zhhxu90
1
gravatar for Bjoern Gruening
3.9 years ago by
Bjoern Gruening5.1k
Germany
Bjoern Gruening5.1k wrote:

I may be completely wrong, but I would say that you can simply put both files together. To do this you can use the tool "Concatenate datasets tail-to-head".

Cheers,

Bjoern

ADD COMMENTlink written 3.9 years ago by Bjoern Gruening5.1k

Thanks Bjoern, I will take a shot.

ADD REPLYlink written 3.9 years ago by zhhxu90
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