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• Tophat Error.
  Hi Everyone. Somebody knows what this error message means: An error occurred running this job:S...
• NGS mapping via Bowtie on paired end trimmed files is failing
  Hi Galaxy support, I have ran paired end files for a staphylococcus aureus genome (Illumina Mis...
• Enhanced bowtie mapper
  Hi all, I use tool called 'Enhanced Bowtie Mapper', and want to map my input file which is FASTQ...
• Aligning S. aureus reads to a reference genome
  Dear colleagues, I am currently trying to aligning reads from a dual RNA-seq experiment to the S...
• Output files: "genome.*.bt2" file. What is it?
  Hi everyone, I ran Bowtie2, mapping my MiSeq reads against a plasmid (8kb) I had to find out if ...
• error tool Bowtie2
  Goodmornig, I have a problem with Galaxy, I am using Bowtie2 tool and the output at the end of a...
• Tophat error: An error occurred with this dataset
  Hi, I am new to RNA-seq data analysis and want to identify differentially expressed genes from ...
• Reference genome build (hg19 installation)
  Hi guys. So I basically deposited my data (hg19 , .bam format) onto my local server and when i n...
• Error for running Tophat in GALAXY
  Hello, I was trying to use Tophat of GALAXY (run by my university) to perform mapping of my RNA-S...
• Hisat error: 'NoneType' object has no attribute 'file_name'
  Hi, I create a workflow using local galaxy. PE RNA-Seq data after trimming are mapped against ge...
• Tophat Mapping And Cufflinks Output Issues
  Hello Galaxy Users- I've been using the Main Galaxy server to work on an RNA-Seq project for a n...
• BWA for SOLiD
  Hello: I am receiving an error trying to perform the "Manipulate FASTQ" on my .fastq file. My or...
• Error Occurred When Converting The Solid Output To Fastq
  Hi, when I converted the solid output to fastq, the galaxy always complained the following error....
• TopHat: Error: Couldn't build bowtie index with err = 1
  Hi I'm trying to run TopHat on a Cloudman Galaxy instance and I keep getting the below error. I'...
• [Fwd: Re: Error Occurred When Converting The Solid Output To Fastq]
  Subject: Re: [galaxy-user] error occurred when converting the SOLID output to fastq Date: Wed,...
• Repeated Error With Tophat
  Hello- I am trying to use Galaxy to investigate gene expression differences among datasets. The...
• Generate Pileup Not Working?
  Hi, I am trying to do variants call with "generate pileup". My steps where: 1. BWA 2. select only...
• Wig To Bigwig Error
  Hi, I have hit a brick wall when trying to convert wig files from the GEO to bigwig files. Each...
• TopHat align summary
  Hello, I am using galaxy to analyze RNA seq of 100bp sing end data, sequenced with Illumina 2500...
• Error message in Tophat
  I am receiving the message "an error occurred setting the metadata for this dataset" on all jobs ...
• (No Subject)
  Hi, I have hit a brick wall when trying to convert wig files from the GEO to bigwig files. Each...
• Depth Of Coverage Error
  Hi, I am using the "Depth of coverage on BAM files" tool from the NGS: GATK Tools but encounter...
• SAM-to-BAM; an error occurred setting the metadata for this dataset.
  Hi. I am very new to Galaxy and am following a simple workflow for processing FASTQ files: Step ...