Question: (Closed) NCBI Standalone BLAST
gravatar for ab555
8 months ago by
ab5550 wrote:

I made a fasta format database and am attempting to query another fasta format nucleotide sequence against it on a standalone BLASTn through CLI. The format of the command is so:

blastn -query myfile.fasta -db databsefile.fa

the directory for terminal is same as that of the fasta files and PATH variable is set to the same directory as well (where database resides). The error upon execution is very persistent:

BLAST Database error: No alias or index file found for nucleotide database

I need all possible hacks or tricks around this situation since it is pretty perplexing. All answers welcome! Heads up: Changing blastdb variable didn't work

ADD COMMENTlink written 8 months ago by ab5550

Hello ab555!

We believe that this post does not fit the main topic of this site.

Hello - This forum is focused on using Galaxy. For general bioinformatics questions, please try

For this reason we have closed your question. This allows us to keep the site focused on the topics that the community can help with.

If you disagree please tell us why in a reply below, we'll be happy to talk about it.


ADD REPLYlink written 7 months ago by Jennifer Hillman Jackson25k
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