Question: (Closed) NCBI Standalone BLAST
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ab555 • 0 wrote:
I made a fasta format database and am attempting to query another fasta format nucleotide sequence against it on a standalone BLASTn through CLI. The format of the command is so:
blastn -query myfile.fasta -db databsefile.fa
the directory for terminal is same as that of the fasta files and PATH variable is set to the same directory as well (where database resides). The error upon execution is very persistent:
BLAST Database error: No alias or index file found for nucleotide database
I need all possible hacks or tricks around this situation since it is pretty perplexing. All answers welcome! Heads up: Changing blastdb variable didn't work
Hello ab555!
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Hello - This forum is focused on using Galaxy. For general bioinformatics questions, please try https://www.biostars.org/.
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