So I'm trying to create a metagenomic workflow, but I can't seem to get the formatting correct. I uploaded a FASTA file, then used the FASTA Manipulation/FASTA width formatter (set to 0) to get the nucleotide strings for each identifier/prefix on a single line. I then attempted to use the NGS QC and Manipulation/Rename Sequences tool, but I get an error stating:
fastx_renamer: Invalid input: This looks like a multi-line FASTA file.
Line 7 contains a nucleotides string instead of a '>' prefix.
FASTX-Toolkit can't handle multi-line FASTA files.
Please use the FASTA-Formatter tool to convert this file into a single
If I used the FASTA width formatter and set to 0 so that the prefixes and strings are alternating every line, what's the issue?