Question: Assembly of metatranscriptomic data with reference
gravatar for Aragion
22 months ago by
Aragion0 wrote:

Hello. I have a set of reads produced by metatranscriptomic cDNA sequensing of the total RNA sample. I need to assemble the RNA-virus genome, which was detected in this sample by the PCR-analysis, using reference sequence from GenBank and the reads. I have never encountered such a problem so can you give me some advise on how to perform this task.

assembly • 509 views
ADD COMMENTlink modified 22 months ago by Jennifer Hillman Jackson25k • written 22 months ago by Aragion0
gravatar for Jennifer Hillman Jackson
22 months ago by
United States
Jennifer Hillman Jackson25k wrote:


This publication is one that describes the process (and compares different assembly tools/methods):

Trinity is wrapped for use in Galaxy. It is available at in the tool group "NGS: Assembly". Or you can install the tool into a local/cloud Galaxy of your own.

Thanks! Jen, Galaxy team

ADD COMMENTlink written 22 months ago by Jennifer Hillman Jackson25k
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