Question: RNA-seq result after cuffdiff with no significance.
gravatar for jlzheng
10 months ago by
jlzheng0 wrote:

I analysed samples(two replicates for each group) with overexpression of my interest, I find that overexpression is successful compared from OE to control, but all q_value comes out from cuffdiff are higher than 0.05 and shows no significance including the overexpression gene. which FPKM differs from 26 in control to 3200 in OE. How come this result could be caused? Is p_value significant for analysis?

test fpkm q-value p-value cuffdiff • 517 views
ADD COMMENTlink modified 10 months ago by Jennifer Hillman Jackson25k • written 10 months ago by jlzheng0
gravatar for Jennifer Hillman Jackson
10 months ago by
United States
Jennifer Hillman Jackson25k wrote:


The test statistic will explain why the data was not considered significant. The other values describe this metric. All is explained here:

Galaxy tutorials for RNA-seq:

Thanks, Jen, Galaxy team

ADD COMMENTlink written 10 months ago by Jennifer Hillman Jackson25k
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