Hello all, I have got some RNAseq results back from a group of patients and a group of controls. I ran DESeq2 to see which genes where significantly differential expressed. When I looked at the PCA plots generated in the DESeq2 on galaxy it didn't group perfectly and I was wondering if it is possible to include Co-Variants like ages ect in the analysis? any ideas???
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Question: co-variance in DESeq2
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dc592 • 0 wrote:
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modified 12 months ago
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Jennifer Hillman Jackson ♦ 25k
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12 months ago by
dc592 • 0
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Jennifer Hillman Jackson ♦ 25k wrote:
Hello,
For covariant analysis in Deseq2, multiple top-level factors can be specified and populated with sub-factor levels and counts.
Tutorials:
One top-level factor containing two sub-factor levels: https://galaxyproject.org/tutorials/nt_rnaseq/#perform-differential-gene-expression-testing
Two top-level factors each containing two sub-factor levels: https://galaxyproject.github.io/training-material/topics/transcriptomics/tutorials/ref-based/tutorial.html#analysis-of-the-differential-gene-expression
Hope that helps! Jen, Galaxy team
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