Question: Inconsistent Cuffdiff Output-Corrected Question
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gravatar for i b
6.3 years ago by
i b • 330
i b • 330 wrote:
Dear all, I have two samples, treated and untreated. I ran cufflinks and had the following FPKM: treated: 884532 untreat.: 130247 I then ran cuffdiff and look at the transcript diff. expression values: treated: 33 untreat.:2.9 significant:yes Strangely for another transcripts I have the following FPKM: Cufflinks: treat_:0 untreat_: 0 Cuffdiff values: trat_4.4 untreat_0.2 significant: yes How this can be possible? I had similar results with other transcripts where one one of the two had 0 cufflinks, and the other one very high but the cuffdiff come out with NO TEST or OK but not significant thanks, ib
rna-seq cufflinks • 984 views
ADD COMMENT • link • modified 18 months ago by vm.higareda • 0 • written 6.3 years ago by i b • 330
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gravatar for vm.higareda
18 months ago by
vm.higareda • 0 wrote:

Hello I have the same trouble, Did you resolve or understand the problem?

ADD COMMENT • link written 18 months ago by vm.higareda • 0

this question is almost 5 years old, I recommend creating a new one.

ADD REPLY • link written 18 months ago by Martin Čech ♦♦ 4.9k

Please see the reply in this thread: https://biostar.usegalaxy.org/p/4683/#23418

ADD REPLY • link written 18 months ago by Jennifer Hillman Jackson ♦ 25k
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