I have relatively large trimmed FASTQsanger files that I want to align via Star and eventually do differential expression analysis on. However, I keep getting the error "Remote job server indicated a problem running or monitoring this job.". The FASTQsanger files are paired-end and the following sizes (R2 of the samples being the same size):
1-R1 (12.5 GB)
2-R1 (21.1 GB)
3-R1 (29.5 GB)
4-R1 (15.0 GB)
Are these files too large for the server to process correctly? If so, should I separate them into smaller files and then combine the mapped Star bam file? How would I do that exactly? Thank you.