Hi... I am unable to run the files after uploading. The uploading is done but on the left side one clock like sign exists and when I am clicking on that it shows the file is waiting to run.Please help and guide so that I can use tophat and also in tophat section the second option RNA-Seq FASTQ file when I am selecting multiple data sets it doesn't show anything, so how to select multiple files in that..????
A gray dataset indicates a job is queued (waiting to run). Once loaded, datasets with the expected datatype will be present in the select list on tool forms. Tophat plus most tools that expect
fastq data should be confirmed to be in
fastqsanger format and then assigned that datatype (during upload or after, before using mapping tools).
Galaxy tutorials, including how to upload, format, and manipulate data:
Also see the help for checking/setting datatype metadata for files in the Support FAQs:
Hope this helps! Jen, Galaxy team