Question: no fastqsanger dataset available after successfully uploading fastqfile
0
gravatar for xue.zhang
22 months ago by
xue.zhang • 10
xue.zhang • 10 wrote:

I uploaded the fastq file to galaxy successfully and can view its detail. When I use 'compute quality statistics' tool under 'NGS: QC and manipulation', I cannot select my fastq file from 'Library to analyze' drop-down list because it shows 'no fastqsanger dataset available'. Does anybody know what's going wrong here?

Thanks, Xue

rna-seq galaxy • 703 views
ADD COMMENT • link • modified 22 months ago • written 22 months ago by xue.zhang • 10
1
gravatar for xue.zhang
22 months ago by
xue.zhang • 10
xue.zhang • 10 wrote:

I know the answer: Use the tool NGS: QC and manipulation -> FASTQ Groomer to convert the file to standard fastq format

ADD COMMENT • link written 22 months ago by xue.zhang • 10

Good answer! Adding a few more details in case needed.

General information about how tool forms work is here: https://wiki.galaxyproject.org/Support#Tool_doesn.27t_recognize_dataset

Details about how-to assign the correct datatype for fastq data is here: https://wiki.galaxyproject.org/Support#FASTQ_Datatype_QA

ADD REPLY • link modified 22 months ago • written 22 months ago by Jennifer Hillman Jackson ♦ 25k
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