Question: Bowtie
0
gravatar for Sher, Falak
7.2 years ago by
Sher, Falak80
Sher, Falak80 wrote:
Hi Experts, I have single end Illumina reads from ChIP-Seq experiment. The files have encoding Illumina 1.5, and the sequence length is 76bp. After basic FastQc I want to map the sequences using Bowtie. My question is: do I need to split my reads (farward and backward) before running mapping tool? In one of Galaxy screen shorts reads are spitted while not in the other. Thank you in advance, F
alignment bowtie • 823 views
ADD COMMENTlink modified 7.2 years ago by Jennifer Hillman Jackson25k • written 7.2 years ago by Sher, Falak80
0
gravatar for Jennifer Hillman Jackson
7.2 years ago by
United States
Jennifer Hillman Jackson25k wrote:
Hello Falak, Single your data is single end, there should be no forward/reverse sequence data to split, you can just run Bowtie in a single run. Hopefully this helps, Best, Jen Galaxy team -- Jennifer Jackson http://usegalaxy.org http://galaxyproject.org
ADD COMMENTlink written 7.2 years ago by Jennifer Hillman Jackson25k
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 16.09
Traffic: 155 users visited in the last hour