Question: RNA STAR FATAL PARAMETER ERROR: limitGenomeGenerateRAM=31000000000is too small for your genome
0
gravatar for Kaylinnette.Pinet
5 months ago by
Kaylinnette.Pinet0 wrote:

I am running RNA STAR to align RNA seq reads to the X. laevis transcriptome, which I uploaded from NCBI, but this is a very large genome of about 3.3x10^9. The current genome size limit for RNA STAR is for the human genome. Is there any workaround for this issue?

rna-seq alignment • 282 views
ADD COMMENTlink modified 5 months ago by Jennifer Hillman Jackson23k • written 5 months ago by Kaylinnette.Pinet0
1
gravatar for Jennifer Hillman Jackson
5 months ago by
United States
Jennifer Hillman Jackson23k wrote:

Hello,

The genome is too large to run with this tool at http://usegalaxy.org. Alternatives ways to use Galaxy are explained here: https://galaxyproject.org/choices/

We will be adding native indexes for this tool in the near future.

Thanks! Jen, Galaxy team

ADD COMMENTlink written 5 months ago by Jennifer Hillman Jackson23k
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 16.09
Traffic: 89 users visited in the last hour