Question: Tophat ends without error message
0
gravatar for jling
3.6 years ago by
jling70
United States
jling70 wrote:

Hi Galaxy,

I've been trying to use Tophat for the past week but I keep encountering an issue where the run appears to begin (turns yellow) and runs for a few hours but then reverts back to gray and says waiting to run (no error message).

After maybe 8 tries one of my samples worked but the other one still has this issue. I've tried reuploading the fastqsanger files and retrying but it doesn't appear to help.

Could someone tell me what is going on with Tophat? Does each Tophat run need to enter the queue twice or something?

Thanks

ADD COMMENTlink modified 3.6 years ago • written 3.6 years ago by jling70
2
gravatar for Nate Coraor
3.6 years ago by
Nate Coraor3.2k
United States
Nate Coraor3.2k wrote:

Hi,

Your job has reached the time limit in our default queue and has been resubmitted to the Stampede supercomputer at TACC. The wait time on Stampede is variable, but is typically less than 1 day.

I'll be putting up a bit of documentation shortly on how long the time limit for each tool is and some tips on how to get your jobs to run as quickly as possible. In the short term, if you use the job resource selector at the bottom of the tool form to select Stampede for jobs that you know will run longer than the time limit, you can avoid the initial queued -> run -> queued step.

ADD COMMENTlink written 3.6 years ago by Nate Coraor3.2k

Documentation is updated here: https://wiki.galaxyproject.org/Main

ADD REPLYlink written 3.6 years ago by Nate Coraor3.2k
0
gravatar for Jennifer Hillman Jackson
3.6 years ago by
United States
Jennifer Hillman Jackson25k wrote:

Hello,

What server are you working on? The public Main Galaxy at http://usegalaxy.org or somewhere else?

If another public Galaxy server, contacting the administrators of that instance is best. If using a local or cloud, the issue could be a resource or configuration issue - if this is it - please send more details and we can try to help troubleshoot.

Best, Jen, Galaxy team

ADD COMMENTlink written 3.6 years ago by Jennifer Hillman Jackson25k
0
gravatar for jling
3.6 years ago by
jling70
United States
jling70 wrote:

I'm using the public server, yes (http://usegalaxy.org)

I just upload my fastqsanger files via FTP and run Tophat. I'm very familiar with Galaxy and have done this many times in the past, but for some reason I've been having trouble this past week.

ADD COMMENTlink modified 3.6 years ago • written 3.6 years ago by jling70
0
gravatar for jling
3.6 years ago by
jling70
United States
jling70 wrote:

I see, I'll change the Job Resource next time. Thanks

ADD COMMENTlink modified 3.6 years ago • written 3.6 years ago by jling70
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 16.09
Traffic: 181 users visited in the last hour