Question: Noisy Histone ChIP Seq data
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VY • 120 wrote:
Hi
I got back some data in the format of aligned BAM files for three histone IPs I did on H3K27me3, H3K4me3 and H3K36me3.
After doing peak calling and processing bed files in IGB, when I generate Depth graphs I see that there is alot of noise, for examples my H3K27me3 gives spikes throughout the gene rather than a peak as you would expect. Also H3K36me3 for example has peaks in the middle of two random genes, which you wouldn't expect as its an elongation mark.
My question is, what are your suggestions to minimize the noise and at what stage?
Thanks
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modified 3.7 years ago
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Jennifer Hillman Jackson ♦ 25k
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3.7 years ago by
VY • 120
