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• Biological Replicates on galaxy/ DeSeq analysis
  Hi, I am doing RNA seq analysis and I want to preform Deseq. I have 2 replicates for each condit...
• Normalization of RNA-seq data
  Hi, I am wanting to normalize my RNA-seq data without running a differential gene expression on...
• RNA-seq data to check impact of PCR duplicates
  I have aligned my reads using HISAT, filtered and merged my data into a BAM file. I have tried re...
• RNA-seq analysis in Galaxy
  I am doing RNA-seq analysis for several mouse samples and I encounter problems during differentia...
• Differential expression analysis with limma-voom
  Hi there: I have been following your tutorial **"RNA-Seq reads to counts"** https://galaxyproject...
• RNA-SEQ analysis tutorial
  Hi, i am a new user to Galaxy and interested to start RNA-SEQ analysis. for this purpose, i need ...
• HTSeq-count, maximum buffer size exceeded
  Hi all, I am new of RNA-seq analysis. I am running HTSeq-count on galaxy with defaut parameters....
• Query Regarding Rna-Seq Data Analysis
  Hi, I am having a fastq file for my RNA data analysis in my computer. How to make it available f...
• Rna-Seq Analysis Withtout Reference Genome
  Hi all, I'm about to start doing gene expression analysis but i don't have a reference genome. I...
• Counts Of Mapped Reads For Each Gene?
  Hi Jen and other galaxy-users, I am analyzing our RNA-seq data. First, I mapped the RNA-seq da...
• HG38 .gtf file
  Hello. For practise, I am running an RNA-seq analysis on some of the RNA-seq data from Illumina ...
• Counting Rna-Seq Reads Per Class.
  Hi All, I have been trying to count the number of RNA-seq reads that fall into the various Cuffli...
• Htseq-count: How to add id attribute and additional attributes in Galaxy (RNA-seq)
  Hello, I am working with a bacterial genome. I'm using DESeq2 for differential gene expression an...
• Bacteria RNA-Seq Analysis - htseq 0 counts
  Hello, I am new to bioinformatics, and am looking at the expression of bacteria genes under diff...
• HTSEQ count error
  Hello, This is my first rna-seq data analysis project and I am using Galaxy to analyze different...
• Can Galaxy be used for straned RNA seq analysis
  My question is as the title. I am wondering whether galaxy can be used for the stranded RNA-seq a...
• annotations with genome of reference
  hi, Dears I am working with RNA-seq and i dont understand how create a de novo transcriptome usi...
• How to add my reference genome to use RNA STAR in usegalaxy.org
  "If your genome of interest is not listed, contact the Galaxy team (--genomeDir)" It is my first ...
• input file types to DESeq on the cloud
  Hi there, I've had a go at using the Ratsch lab DESeq tool but it seems to require your input fi...
• "History could not be imported. Please contact a Galaxy administrator" error
  I am having my students use the RNAseq tutorial data from jeremy's post here: https://usegalaxy....
• DE analysis of miRNA after mapping with miRDeep2 quantifier
  After small RNA sequencing I performed adapter, quality, and length trimming on fastq files. I th...
• Rna Seq Analysis
  I am new to Galaxy and I am not sure whether these topics were discussed earlier. I followed the ...