Question: (Closed) annotations with genome of reference
gravatar for pilar.rodriguez.becerra.2
19 months ago by

hi, Dears

I am working with RNA-seq and i dont understand how create a de novo transcriptome using a genome of refference. I work the annotations with a genome of reference of Chrysemys pucta bellii. I was search the annotation in NCBI and I find two folders in NCBI, an folder in format gff and other in format fasta. In the Folder GFF there are two files. one file named Scaffold in format gff3 and other named top level also in format gff3. I do my analysis with this folders but the tool Deseq does not work.I need to know how folder i need use for do my work

can you help me pleasse


rna-seq • 389 views
ADD COMMENTlink written 19 months ago by pilar.rodriguez.becerra.20

Hello pilar.rodriguez.becerra.2!

Questions similar to yours can already be found at:

We have closed your question to allow us to keep similar content in the same thread.

If you disagree with this please tell us why in a reply below. We'll be happy to talk about it.


PS: Please see the related post and link out for help with Deseq, direct from one of the tool authors.
ADD REPLYlink written 19 months ago by Jennifer Hillman Jackson25k
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