Question: for viral metagenomics
0
gravatar for reach2anamika
4.4 years ago by
United States
reach2anamika10 wrote:

I want to mapmy illumina reads to the viral genome database. Please give me the workflow for this. 

In the Blastx there is an option to map only to the viral database within the nr database.

galaxy • 935 views
ADD COMMENTlink modified 4.4 years ago by Jennifer Hillman Jackson25k • written 4.4 years ago by reach2anamika10

Are you using your own Galaxy instance or a public one?

ADD REPLYlink written 4.4 years ago by Bjoern Gruening5.1k

I am using the public one.

ADD REPLYlink written 4.4 years ago by reach2anamika10
0
gravatar for Jennifer Hillman Jackson
4.4 years ago by
United States
Jennifer Hillman Jackson25k wrote:

Hello,

The Megablast tool (blastn) on the public Main Galaxy instance at http://usegalaxy.org has a fixed set of target databases.

However, you can install and use either Megablast or BLAST+ on a suitable local or cloud Galaxy instance with the target database of your choice. Here are some links to get started:

http://usegalaxy.org/toolshed
http://wiki.galaxyproject.org/BigPicture/Choices

Good luck! Jen, Galaxy team

ADD COMMENTlink written 4.4 years ago by Jennifer Hillman Jackson25k

Thank you.I will try them.

ADD REPLYlink written 4.4 years ago by reach2anamika10
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