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• Issues On Rnaseq Since The Changeover
  My histories seem to be stopping their processing around the Tophat-cuffmerge steps since the cha...
• Problems Processing Histories Existing On Galaxy Before The Changeover
  Is anyone who had developed histories on Galaxy online before the changeover getting red error me...
• Failed Tophat Processing Steps
  Hi, I submitted a sample for Tophat processing on the main Galaxy instance and it seems to me th...
• Share History - Error
  Hi, Today was my first time to present a workshop. Was about RNAseq and i used the webtool Galax...
• Problems With Tophat
  Hello, I have successfully used the public server for processing RNAseq data but I have run into...
• Mark Duplicates - MAPQ = 0 and Value was put into PairInfoMap more than once
  Dear Biostars Galaxy team, I'm relatively new to using the Galaxy online platform and have been ...
• "History could not be imported. Please contact a Galaxy administrator" error
  I am having my students use the RNAseq tutorial data from jeremy's post here: https://usegalaxy....
• After loading up RNAseq data, what to do next?
  Hi, Could an expert please give me a lay instruction on below matters? -- Q1: I have uploaded ...
• Problems In Transitioning From Tophat To Cuffdiff
  After successfully using RNAseq software in Galaxy online for about 10 different datasets to jus...
• RNAseq data mapping to reference genome
  Hello, I have some RNAseq Illumina fastq data. I would like to align it to the referece genome o...
• Failed Uploads from EBI SRA to import to Galaxy
  I am unable to load anything from EBI SRA. I have not been able to for a few days. It starts, and...
• bwa-MEM fails to execute with reference sequence (hg19.fasta) from history
  Hi, I have been trying to use custom reference genome hg19.fasta and choosing the option to buil...
• Graduate student learning to do RNAseq analysis
  Hi, I am trying to do RNAseq analysis using Galaxy. I have tophat files which i used to run the c...
• RNAseq data to be processed in two ways: (i) mapping to de novo Trinity-based transcriptome and (ii) mapping a relatively new genome
  Hello all, I am new to RNAseq data and learning this process step by step, so I have a few quest...
• Paired-end Reads trimming
  Hey there. I am starting to analyse some RNAseq paired-end data. At the pre-processing step I go...
• Cufflinks options to get similar results
  Hello everyone, I'm trying to reproduce the results from a dataset from RNAseq I found on GEO. A...
• Estimating Galaxy On The Cloud Needs For Rnaseq
  Questions: I have been running sets of data for cufflinks/cuffdiff analysis on Galaxy online--I ...
• Same Rnaseq Samples Give Different Fpkm Values When Run Against Different Samples
  Hello, I am sure this has come up before and maybe I missed the answer. If in using cuffdiff I r...
• Can limma's voom take normalized counts as input?
  Hi,      I am currently analyzing some RNASeq data from TCGA - which is data that has been proce...
• Very Slow galaxy Main server
  Dear Sir or Madam, I am a user of the online galaxy platform for analysing the RNAseq data. From...
• Problem With Bam And/Or Bai Files
  Hello Galaxy Team,   I have been using Galaxy for SNP detection for with great success. Basically...
• Galaxy instance on jestream- cluster error for downloaded files
  I am using a galaxy instance on Jetstream but have run into a problem getting data files into gal...