Question: Bed File To Gene List
gravatar for Christopher Futtner
7.0 years ago by
Christopher Futtner20 wrote:
Can anyone point me to a good workflow to convert my ChIP-seq data intervals to gene names. My peaks fall both within genes and within intergenic areas. Thanks, Chris Christopher Futtner, Ph.D. Dept. of Surgery Duke University Durham, NC 27710 email: phone: 919-684-1183
chip-seq • 4.2k views
ADD COMMENTlink modified 7.0 years ago by Jennifer Hillman Jackson25k • written 7.0 years ago by Christopher Futtner20
gravatar for Jennifer Hillman Jackson
7.0 years ago by
United States
Jennifer Hillman Jackson25k wrote:
Hello, There is no general workflow, primarily because there is no single source of gene annotation and some formatting may be necessary. But once that part is resolved, using a single tool in "Operate on Genomic Intervals" can most often assign gene names to query genome regions. The first step is to obtain a dataset that has gene names assigned to genome regions. This data should be based on the same reference genome as your peak intervals. Sources can vary by species and reference genome build. For most model organisms, a source can be found in the set of linked projects under "Get Data" and the dataset directly imported into Galaxy. For certain genomes at UCSC, the tool "Operate on Genomic Intervals -> Profile Annotations" is a quick way to see which UCSC Gene tracks are associated. The RefSeq Genes track would be one example. Once in Galaxy, use the tools in "Operate on Genomic Intervals" to compare your peak intervals with gene intervals and link in gene names. "Join" would be the simplest option. Help is located on each tool's form, including links to the wiki and screencasts, but also directly here: Hopefully this helps, Best, Jen Galaxy team -- Jennifer Jackson
ADD COMMENTlink written 7.0 years ago by Jennifer Hillman Jackson25k
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