Question: Trouble In Extracting Species On Maf Imported From Ucsc
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gravatar for 4urelie K
7.1 years ago by
4urelie K10
4urelie K10 wrote:
Hi, When I import a multiz46way maf alignement from UCSC, if is is a whole chromosome, then when I try to "Filter MAF blocks by Species" the list of species does not appear. If I import a small region, it works... Is it a question of size? Although it seems OK when I look at it, could it be a problem in the maf format that I could solve? Thank you, Aurelie
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ADD COMMENTlink modified 7.1 years ago by Jennifer Hillman Jackson25k • written 7.1 years ago by 4urelie K10
0
gravatar for Jennifer Hillman Jackson
7.1 years ago by
United States
Jennifer Hillman Jackson25k wrote:
Hi Aurelie, Would you have time to share a history link containing the two types of datasets for me? Use "Options -> Share or Publish", generate the link, then email it back to me directly. Please note the dataset(s) with the issue if not obvious (or make a note about it in the "info" field of the dataset). I will watch for your feedback, Jen Galaxy team -- Jennifer Jackson http://usegalaxy.org http://galaxyproject.org/wiki/Support
ADD COMMENTlink written 7.1 years ago by Jennifer Hillman Jackson25k
Hi Aurelie, The dataset from the UCSC Table browser did not transfer completely, which lead to the other errors. There is a limit to the number of rows of data that can be extracted, to Galaxy or any other type of download. It is around ~100k, but it depends on the data type. The UCSC group would have the details. To access human hg19 MAF data in Galaxy, use these tools, then proceed with the rest of your analysis: - Extract Pairwise MAF blocks given a set of genomic intervals - Extract MAF blocks given a set of genomic intervals Thanks for sending the link and hopefully this option will work for your project, Best, Jen Galaxy team -- Jennifer Jackson http://usegalaxy.org http://galaxyproject.org/wiki/Support
ADD REPLYlink written 7.0 years ago by Jennifer Hillman Jackson25k

What if the task is to do this with mm10, for which you do not have MAF data in Galaxy (and importing it via table browser or file download/upload does not work)??? 

ADD REPLYlink written 4.0 years ago by dvanic0

Hello,

There are two choices for loading large data files from other public sources:

1. download the files directly from UCSC, then load those into Galaxy via FTP

2. paste the URL of the data file directly into the Upload tool (at the top of the tool panel) or into the Get Data -> Upload file tool.

The first will show progress. And both accept most compressed file formats. Note that this will only work with public datasets (UCSC's are public). 

This details for how to use the second tool are in our wiki here. 
http://wiki.galaxyproject.org/Support#Loading_data

The first tool has documentation that we'll be linking in, but it is fairly intuitive to use, especially after reviewing how the second one works

Thanks, Jen, Galaxy team

ADD REPLYlink written 4.0 years ago by Jennifer Hillman Jackson25k
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