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• Fastq Splitter Empty And Fastq Manipulator Doesn'T Work
  I am having the same issue as this user: http://user.list.galaxyproject.org/FASTQ-splitter-produ...
• Too few reads after VarScan on RNA-Seq data?
  Hi all, I have been trying to initiate a protocol to call SNPs in RNA-Seq data, but have had a f...
• Combining The Paired Reads From Illumina Run
  Hi, I have two fastq files with the forward(/1) and reverse(/2) paired reads. The reads are not ...
• Can't tell which is the forward/reverse FASTQ splitter
  I ran the FASTQ splitter on some data, and now I have two files, but I don't know which file is t...
• Need Help To Split Paired-End Dataset
  I am new to the NGS analysis. I need help to solve this problem. As shown in my previous emial...
• (No Subject)
  I am new to the NGS analysis. I need help to solve this problem. As shown in my previous emial...
• Considerations for trimming poor quality Illumina MiSeq paired end reads
  I have generated fastq files from Illumina MiSeq for bacterial genome sequencing. My reads are 2...
• Separating (F and R) reads uploaded from NCBI SRA & mapping them using BWA-MEM
  Can someone explain to me the best way to prepare NCBI SRA data for subsequent mapping to a cust...
• Splitting Biased Paired End FASTQ data
  The FASTQ splitter in Galaxy warns that the tool only works if Paired-end data has equal length r...
• Forward and Reverse reads from paired end sequencing
  The fastq dataset we have after illumina whole genome sequencing is in the form of two files - fo...
• Convert multiple FASTQ files into one BAM file
  Hello guys! I recently started working with NGS analysis and I may have a stupid question to ask....
• Merging Fastq Files
  Hi I have received Illumina paired-end genome sequence data as a .tar file. When unpacked the dat...
• Extract reads in FASTQ/A format from NCBI SRA
  Hi. I downloaded an SRA accession. In the results, the spots were separated but is in the same fi...
• Making paired-end reads the same length
  I have paired-end data (in two separate files).  I have groomed my files, and would like to filte...
• Analysis of forward and reverse reads
  Hello. Newbie here: I have recently done some chip-seq, and for each sample, I have different ...