Hello guys! I recently started working with NGS analysis and I may have a stupid question to ask.
Through lab analysis (nextseq 500 Illumina) using paired-end sequencing, I produce 8 fastq files, 4 forward and 4 reverse from the same sample. Ideally the 4 forward should be identical as well as the 4 reverse files (but they never are).
I want to convert the 8 FASTQ files into 1 BAM file. What is the procedure I need to follow using galaxy tools? I imagine that the 4 forward files should somehow become 1 file. Should I use the concatenate tool?
Thank you very much in advance!! Please save me!