Question: Bedgraph to bigwig: Matched on Error
0
gravatar for pcng
3 months ago by
pcng0
pcng0 wrote:

Hello Biostars,

I've been encountering this error with the bedGraphToBigWig tool Wig/BedGraph-to-bigWig converter (Galaxy Version 1.1.0) at http://usegalaxy.org with the SacCer3 genome build.

The 7293 line referenced is the very first coordinate containing one (line 2). Using the UCSC wrapper script bedGraphToBigWig.dms generates a bigwig file, but is unviewable in the UCSC Genome Browser or a JBrowse instance.

Both the chromSize file and the bedGraph file use Roman numeral notation, but substituting arabic for roman numerals for a small test sample also generates a 'Matched on Error' similar to the one below.

7293 7311 doesn't intersect 7293 7293, chromId 0 chromSize 230218 Internal error bbiWrite.c 264 Fatal error: Matched on Error Error running wigToBigWig.

Please let me know if I need to clarify my problem further. Thank you!

Cheers, Patrick

ADD COMMENTlink modified 3 months ago by Jennifer Hillman Jackson25k • written 3 months ago by pcng0
0
gravatar for Jennifer Hillman Jackson
3 months ago by
United States
Jennifer Hillman Jackson25k wrote:

Hello,

This prior Q&A covers the same type of issue: https://biostar.usegalaxy.org/p/12499/

All Support FAQs (cover expected formatting, chromosome mismatches, and more): https://galaxyproject.org/support/

Please start here to see if helps to resolve the problem: https://galaxyproject.org/support/chrom-identifiers/

Thanks, Jen, Galaxy team

ADD COMMENTlink written 3 months ago by Jennifer Hillman Jackson25k
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