Question: Galaxy On The Cloud - Cannot Select Fastq Files When Trying To Run Workflow
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gravatar for Amit Indap
7.6 years ago by
Amit Indap60
Amit Indap60 wrote:
Hi Galaxy, I am a newbie to Amazon EC2 and have been carefully following the steps in the screencast. I am able to upload two fastq files from the s3 bucket: http://s3.amazonaws.com/heteroplasmy/F4-bM4-1.fastq http://s3.amazonaws.com/heteroplasmy/F4-bM4-2.fastq I am also able import the published workflow http://s3.amazonaws.com/heteroplasmy/Galaxy-Workflow-mt_analysis_0 .01_strand-specific_(fastq_double).ga But when it comes to running it, I cannot select the fastq file from the drop down menu. I am able to view them on my GC instance, since I imported them successfully, but am at a loss as to why I can't select them from the drop down menu in the workflow to begin their alignment. Is it something to do with my security group settings in setting up the EC2 instance? Any assistance you can provide would be great! Thanks, Amit -- Amit Indap
galaxy • 1.3k views
ADD COMMENTlink modified 7.6 years ago by Enis Afgan680 • written 7.6 years ago by Amit Indap60
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gravatar for Enis Afgan
7.6 years ago by
Enis Afgan680
Enis Afgan680 wrote:
Hi Amit, The workflow requires the input data to be in 'fastqsanger' format before being able to run. The files you uploaded from S3 are already in the correct format but this is most likely not set correctly in the metadata. So, click on the pencil icon for each of the datasets in your history and edit the data type by setting it to 'fastqsanger'. Save the changes and try running the workflow. It should work fine then. For future reference, if you decide to upload the rest of the files from the screencast/heteroplasmy study, you can choose the fastqsanger type right on the data upload form and can thus avoid this subsequent step. Enis
ADD COMMENTlink written 7.6 years ago by Enis Afgan680
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