Question: How to choose values in trimmomatic steps ?
0
gravatar for aman.deep13023
14 months ago by
aman.deep130230 wrote:

which one file to choose the adapter sequences to use and what are the value to choose for Maximum mismatch count which will still allow a full match to be performed ? How accurate the match between the two 'adapter ligated' reads must be for PE palindrome read alignment ? How accurate the match between any adapter etc. sequence must be against a read? and in trimmomatic opretion .

galaxy • 1.2k views
ADD COMMENTlink modified 14 months ago by Jennifer Hillman Jackson25k • written 14 months ago by aman.deep130230
0
gravatar for Jennifer Hillman Jackson
14 months ago by
United States
Jennifer Hillman Jackson25k wrote:

Hello,

How to perform QA on your data depends on the fastq content and the downstream analysis to be performed.

Many of the Galaxy tutorials cover QA steps and can be found here: https://galaxyproject.org/learn/

Trimmomatic is one choice for trimming. The tool itself (the algorithms) and parameters (the usage) are described here and most options are implemented in the Galaxy wrapped version of the tool: http://www.usadellab.org/cms/index.php?page=trimmomatic

Hope this helps! Jen, Galaxy team

ADD COMMENTlink written 14 months ago by Jennifer Hillman Jackson25k

Thanks Mam, it is working

ADD REPLYlink written 14 months ago by aman.deep130230
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