I have three large .fastaq files for an Ion Torrent PGM analysis for three species of tuna. I want to align them against a mtDNA template for tuna. I have looked at the Galaxy tutorial but it does not address what I want to. I have done the FastQC runs and for the most part they look good. My understanding is that I am to trim the segments- I am presuming that means the adapters and barcodes, and/or the larger pieces (>200 bases), or something else? I would appreciate any advice, a link to a tutorial, or whatever one feels necessary for me to accomplish my goal. I thank all in advance. Sincerely, Michael Eugene Pugh visiting scientist VA Institute of Marine Science mepugh@vims.edu
Heads up! This is a static archive of our support site. Please go to help.galaxyproject.org if you want to reach the Galaxy community. If you want to search this archive visit the Galaxy Hub search
Question: Newbie wanting to align Ion Torrent PGM data to mitochondrial DNA template
0
mpugh • 0 wrote:
ADD COMMENT
• link
•
modified 17 months ago
by
Jennifer Hillman Jackson ♦ 25k
•
written
17 months ago by
mpugh • 0
0
Jennifer Hillman Jackson ♦ 25k wrote:
Hello,
Help for using Ion Torrent data in Galaxy is in this prior Q&A: https://biostar.usegalaxy.org/p/22071/
If you need more details about protocol best-practises and options, reviewing current publications or asking/reviewing prior questions at a general bioinformatics forum such as https://www.biostars.org/ would be a good choice.
Thanks! Jen, Galaxy team
Please log in to add an answer.
Use of this site constitutes acceptance of our User
Agreement
and Privacy
Policy.
Powered by Biostar
version 16.09
Traffic: 171 users visited in the last hour