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Question: bam to stranded bigwig?
I have a bam file generated from stranded RNA-seq data - is it possible to use galaxy to split this bam file into two bigwig (or similar) files, one for each strand?
18 months ago by
Try the tool DeepTools: bamCoverage. On the tool form, under "advanced options", are settings that can apply strand filters.
Thanks! Jen, Galaxy team
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