Question: Mate_Not_Found after FixMate and filter
0
gravatar for nhs
24 months ago by
nhs0
European Union
nhs0 wrote:

Hi

After applying FixMate and filtering on "isMapped" and "isMatemapped" (and quality and no duplicates) I still have a considerable amount of reads flagged as Mate_Not_Found when running ValidateSam. Mapping to different chromosomes seems not to be the issue judged by Flagstat. How can I get rid of (or salvage) these singletons?

Thanks

medip-seq samtools bam • 330 views
ADD COMMENTlink modified 24 months ago by Jennifer Hillman Jackson24k • written 24 months ago by nhs0
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