Question: Mate_Not_Found after FixMate and filter
gravatar for nhs
2.2 years ago by
European Union
nhs0 wrote:


After applying FixMate and filtering on "isMapped" and "isMatemapped" (and quality and no duplicates) I still have a considerable amount of reads flagged as Mate_Not_Found when running ValidateSam. Mapping to different chromosomes seems not to be the issue judged by Flagstat. How can I get rid of (or salvage) these singletons?


medip-seq samtools bam • 358 views
ADD COMMENTlink modified 2.2 years ago by Jennifer Hillman Jackson25k • written 2.2 years ago by nhs0
Please log in to add an answer.


Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 16.09
Traffic: 87 users visited in the last hour