Question: Mate_Not_Found after FixMate and filter
gravatar for nhs
2.6 years ago by
European Union
nhs0 wrote:


After applying FixMate and filtering on "isMapped" and "isMatemapped" (and quality and no duplicates) I still have a considerable amount of reads flagged as Mate_Not_Found when running ValidateSam. Mapping to different chromosomes seems not to be the issue judged by Flagstat. How can I get rid of (or salvage) these singletons?


medip-seq samtools bam • 433 views
ADD COMMENTlink modified 2.6 years ago by Jennifer Hillman Jackson25k • written 2.6 years ago by nhs0
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