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Question: How to compare two libraries
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gravatar for fradiancona
2.6 years ago by
fradiancona • 20
fradiancona • 20 wrote:

Dear Service,

I load in Galaxy server about 10 histories. Each one is composed of a fastq file and the related intercepts with known genome sequences of my interest (Alu sequences).

Now I would like to compare the samples (histories) considering the regions of my interest and so by quantifying the number of regions per library and the positions of the regions in the genome.

I would expect that a disease genotype is characterized by a different position of the Alu sequences along the genome.

Thank you Francesco
galaxy • 716 views
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modified 2.6 years ago • written 2.6 years ago by fradiancona • 20

What type of data is the fastq? RNA or DNA? From a specific technology?

ADD REPLY • link written 2.6 years ago by Jennifer Hillman Jackson ♦ 25k
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gravatar for fradiancona
2.6 years ago by
fradiancona • 20
fradiancona • 20 wrote:

Fastq is the extension of the file. This file contains about 10 million of reads of DNA sequences of about 100-400bp. In each one of these sequences there is a known sequence. Now, I would like to check where these known sequences are in the genomic DNA. Is there a TOOL by which I could check these insertions/deletions (indel) ?
ADD COMMENT • link written 2.6 years ago by fradiancona • 20
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