2.9 years ago by
The input fastq dataset probably needs to have the "fastqsanger" datatype assigned.
These two wiki sections combined explain how to assign a datatype and equally important - how to make certain that datatype is correct. No one likes to start over - so I recommended first confirming the type, including making modificataions as needed, then executing the tools. If the data is a mismatch for assigned type, unexpected results can occur and not always with the first tool in the pipeline, which is always frustrating (for anyone doing bioinformatics, in Galaxy or through other means).
Support#Dataset_special_cases (section leads with fastq type testing plus how-to for manipulation if needed)
We hope this with your project! Jen, Galaxy team