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Question: Illumina DNA sequence upload problem
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gravatar for lnaren
3.1 years ago by
lnaren • 10
Canada
lnaren • 10 wrote:

The Illumina DNA sequence for single sample we got was 59 (uncompressed), but I learned that the maximum file you can upload by using FTP should be <50G. Is there any other way I can upload the file to Galaxy?
fastq upload ftp • 929 views
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modified 3.1 years ago by Jennifer Hillman Jackson ♦ 25k • written 3.1 years ago by lnaren • 10
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gravatar for Jennifer Hillman Jackson
3.1 years ago by
Jennifer Hillman Jackson ♦ 25k
United States
Jennifer Hillman Jackson ♦ 25k wrote:

Hello,

Split the fasta file locally, then upload the sub-files. Merge once in a history using the tool Concatenate.

Important considerations:

  • Ensure that fasta data is split logically if you wish to use these sub-files independently (meaning that individual fastq records, four lines each, are each fully intact). But, if the data will only be used in the post-Concatenate-merged format with tools, split fastq entries should not trigger problems as these are disposable intermediate datasets.
  • Large input fastq files may exceed the computing resource at http://usegalaxy.org when used with tools. Should this occur, the alternatives outlined here are how to proceed: https://galaxyproject.org/support/tool-error/

Best, Jen, Galaxy team
ADD COMMENT • link modified 16 months ago • written 3.1 years ago by Jennifer Hillman Jackson ♦ 25k
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