Question: Data does not show up on Galaxy
gravatar for gaiusjaugustus
3.3 years ago by
gaiusjaugustus0 wrote:

I uploaded 3 files to  These files are showing up in my history.  I can view them.  They seem to be properly uploaded.


However, when I go to use a tool (any tool, but right now, I am specifically interested in the reverse complement tool, the "Library to reverse-complement:" dropdown is empty.  I verified that it takes fastq files, and that my uploaded files are fastq.


Did I miss a step somewhere?  How can I run my data through this tool?

galaxy • 737 views
ADD COMMENTlink modified 3.3 years ago by Jennifer Hillman Jackson25k • written 3.3 years ago by gaiusjaugustus0
gravatar for Jennifer Hillman Jackson
3.3 years ago by
United States
Jennifer Hillman Jackson25k wrote:


Issues for this server are best directed at the instance owners:

But just as a guess, most fastq data in Galaxy needs to be assigned to the "fastqsanger" datatype before use with tools. It could be that this is the problem. The method to assign the datatype (and re-scale quality scores, if needed) should be about the same across most Galaxy instances, so perhaps try this wiki help and test if it resolves the issues. See sections 2.10 & 2.11

Thanks, Jen, Galaxy team

ADD COMMENTlink written 3.3 years ago by Jennifer Hillman Jackson25k
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