Question: Internal Fail error 500 or file failed
0
gravatar for amh
3.3 years ago by
amh0
Denmark
amh0 wrote:

What am I doing wrong? My files have an extension of .fastq. I am told that these files contain raw data from an Illumina MiSeq sequencer. Therefore, I mark these files as fastqillumina. I am getting an error message. I have tried several times with no success.

I have tried with some success to load these files with the auto-detect but I am not sure if my files will align properly if I don't identify them as fastqillumina. All my research tells me that I must identify my files properly.

Can you help me?

 

galaxy error message • 783 views
ADD COMMENTlink modified 3.3 years ago by Jennifer Hillman Jackson25k • written 3.3 years ago by amh0
0
gravatar for Jennifer Hillman Jackson
3.3 years ago by
United States
Jennifer Hillman Jackson25k wrote:

Hello,

If the sequence data is from a newer Illumina pipeline, then the datatype is most likely "fastqsanger". However, this can and should be tested and the data adjusted if necessary (specifically the quality score scaling). The Galaxy wiki explains "how to":
https://wiki.galaxyproject.org/Support#FASTQ_Datatype_QA

Thanks! Jen, Galaxy team

ADD COMMENTlink written 3.3 years ago by Jennifer Hillman Jackson25k
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