Question: How can i use galaxy to analysis miseq metagenomics data?
0
gravatar for eng_mhas
3.1 years ago by
eng_mhas0
eng_mhas0 wrote:

hi, i am new user in galaxy and in bioinformatic and i have 36 samples of metagenomics data, one sample contain R1 , R2 fastq data and i want to analysis mtagenomics data by galaxy what are the steps i can do to make it and obtain a phelogenetic tree .

the data from miseq machine

R1 (first 2 sequencese) 

@M01784:37:000000000-A7V7D:1:1101:20925:1026 1:N:0:1
NCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTATTCTCCCGGTACTGTCATTATCATCCCGGGTAAA
+
#8ACC,858=CEAFFGDDFFEFFGGGGGGGGGGGGGGGFGGGGGGGGFGGGGGGGGFGGGGGGGGGGF
@M01784:37:000000000-A7V7D:1:1101:9914:1028 1:N:0:1
NTTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACCGTCAAGGTACGAGCAGTTAC
+
#8AC8D5586@FFGGGGGFFGGGGGFE9FGGC6FEGGCFE<,ECFF<FGGGGFFE,CECC<6,:FEF,9FF

R2 

@M01784:37:000000000-A7V7D:1:1101:20925:1026 2:N:0:1
NGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGAGACCTTCGG
+
#8ACBFGGFGGGGGFGGGGFFGGGEGGGGGGGEGGGGGGGGGGDGGGFGGG,CFFGGGGGGGGG@FGGGGG
@M01784:37:000000000-A7V7D:1:1101:9914:1028 2:N:0:1
NGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGGACGGGAAGTGGTG
+
#86CCFGGGGGGGGGGFFGGFCEFGFGGGGGGGGEEEGGGGGGGGGF<FGG?FGGGG:FEFGGGECFFEFE
@M01784:37:000000000-A7V7D:1:1101:13819:1034 2:N:0:1
NGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAGCGAGAACCGGGG
+
#8ACCGGGGGGGGGGGGGGGGG@FGGGGGGEGGGGG7+CGCGCFGGGGGGGFGGGGG:6BF>FFG<<FE+F
@M01784:37:000000000-A7V7D:1:1101:20656:1036 2:N:0:1
NTTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTATTCGTCAGGTACCGTCATCATCGTCCCTGACA
+
#88ACFGGGGGGGGFEGGGGG<CEFCCFGGGGGGGGGGGGGFFGGCFCFGC@CFFGGGGGEFFEC98<FE

How can i do?

 

 

galaxy metagenomics • 1.3k views
ADD COMMENTlink modified 3.1 years ago by Jennifer Hillman Jackson24k • written 3.1 years ago by eng_mhas0
0
gravatar for Jennifer Hillman Jackson
3.1 years ago by
United States
Jennifer Hillman Jackson24k wrote:

Hello,

This publication and supplement should be helpful in getting your pipeline started:
http://usegalaxy.org/u/aun1/p/windshield-splatter

Best, Jen, Galaxy team

ADD COMMENTlink written 3.1 years ago by Jennifer Hillman Jackson24k
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