Hello, I received my raw NextGen sequencing files and am following a lab mate's protocol based on Galaxy. I created my account, then under "Get Data", I uploaded all FASTQ files, specifying type as "fastqsanger" and genome as hp38. For each individual sample, I merged multiple files representing 1 lane into 1 FASTQ file using "Text Manipulation" >Concatenate datasets tail-to-head. My data how looks like the below example. When I tried to proceed to the next step, which prompts to remove possible new/empty lines created between files by using "Filter and Sort" > "that: NOT Matching" and "the pattern: ^$", I couldn't see my merges files in the drop-down box, and am now not sure how to proceed. Any help would be greatly appreciated! Best, Katja
@HWI-1KL153:117:HJL5WBCXX:2:1101:1494:1944 1:N:0:TAGCTT
CTGGTACAGTCCGCTGATGATGGGGTTACACACCTGCTCCAGCTCCTTCCTCTTGTGCTCAAACTCGTCC
+
DA@@DHFFHGHIIICDF1DCE?EEECEHIIIHHIFHHHHH@1GHEHCFHH@FHG?F1DFFE11C<CEFHH
@HWI-1KL153:117:HJL5WBCXX:2:1101:1554:1976 1:N:0:TAGCTT
CGTAGGTTTGGTCTAGGGTGTAGCCTGAGAATAGGGGAAATCAGTGAATGAAGCCTCCTATGATGGCAAA
+
DDDDDIHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHIIIIIIIIIIHIIIIIIIIIIIIII
@HWI-1KL153:117:HJL5WBCXX:2:1101:1825:1907 1:N:0:TAGCTT
TTCCTTCAGCTCAGCAAACTTGCATGCAATGTGAGCCGTGTGGCAATCCAATACAGGGGCATAGCCGGCG
+
DDDDDIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIH
@HWI-1KL153:117:HJL5WBCXX:2:1101:2017:1954 1:N:0:TAGCTT
CCCGCCCATTAATGACACTCCAAGAAGTGTCATGATATATGCGATTCACTTTCAAGTCTTCAGCAAACTA
+
DDDDDIIIIIIIIIIIIIHIIIHIIIIIIIIIIHIIIIIIIIIHIIIIIIIIIIIIIIIIIIIHIIIIII
@HWI-1KL153:117:HJL5WBCXX:2:1101:2737:1946 1:N:0:TAGCTT
GTCAAATGTGAGTCGCCTAGTCTAACAGTAGAGGTAAGTTCAAAGATGAAATGTGATTTGTTCAAGGCTG
+
DDDDDIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII
@HWI-1KL153:117:HJL5WBCXX:2:1101:2594:1994 1:N:0:TAGCTT
GCCAGCAAGCCAGCCCTCATAACCAAAGACAGCTGAACCATGGATTGCAGGTCCAGCAAAATCAAAGTCA
