Question: Repeat identifer in genomic sequence
0
gravatar for mrn0032
4.3 years ago by
mrn003210
United States
mrn003210 wrote:

Is there an option in galaxy to identify repeat motifs?  The track tandem repeat finder, also called simple repeats, provides genomic locations of STRs so the genomic sequence can be extracted.  However it fails to provide the repeat motif.  Is there an option in galaxy to identify repeat motifs?

ADD COMMENTlink modified 4.3 years ago • written 4.3 years ago by mrn003210
0
gravatar for Jennifer Hillman Jackson
4.3 years ago by
United States
Jennifer Hillman Jackson25k wrote:

Hello,

This tool is wrapped in the Tool Shed for use in a production local or cloud Galaxy instance. Cloud is often the choice - be sure to review Amazon's AWS Educational grants program if you are working in an academic/research project or group (turn around for applications can be very quick).

Note that this will require some changes to the tool repository. Specifically, the option "-d data file" needs to be added to the tool wrapper. This will trigger the program to produce an output that reports the nucleotide sequence consensus (motif). More about that option is here: http://tandem.bu.edu/trf/trf.unix.help.html

Request can be made of the wrapper author to include this in the repository version itself (ideally an option on the tool's form, rather then changing the default output. Do this through the Tool Shed (requires login).

Tool Shed repository:
http://toolshed.g2.bx.psu.edu/view/urgi-team/tandem_repeats_finder

Galaxy local/cloud help:
http://wiki.galaxyproject.org/BigPicture/Choices

There are alternate tandom repeat finding utilities in the EMBOSS tool group on the public Main Galaxy server, but I do not believe that these report the motif either.(Just ran a quick test).

Best, Jen, Galaxy team

 

ADD COMMENTlink written 4.3 years ago by Jennifer Hillman Jackson25k
0
gravatar for mrn0032
4.3 years ago by
mrn003210
United States
mrn003210 wrote:

Thanks for the prompt response!

A few follow up questions

By Amazons AWS Educational Grants program you mean the amazon.com in the link I provided right?  It is for an academic research project.  There are legal restrictions I need to be aware of? http://aws.amazon.com/education/

Would I need to install Galaxy locally or on the cloud for this change to work?  Or would the tandem repeat finder owner upload the altered data file for public online use?  Or is that up to the owner of the tool?

ADD COMMENTlink written 4.3 years ago by mrn003210
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