I have a .txt file with >100 lines of the following format (1st column - sequence ID, 2nd - start coordinate, 3rd - end coordinate, 4th - strand; everything separated by TABs):
PA14sr_076 2867353 2867490 +
I am trying to fetch the sequences from the full genome sequence corresponding to these coordinates. I figured out that for this I could convert my file into Interval format. However, I do not understand, what should I use as a CHROM# in this case? As this is a bacterial species, it has only one chromosome anyway.. And when I check the full genome sequence in GenBank - it has no identifiers similar to chromosome or something.
When I am doing "Extract genomic DNA" without providing CHROM# - I get an empty output. Could someone help me, please? Thanks in advance...