Similar posts • Search »

• Trimmomatic on Galaxy
  Hi, How do i use trimmomatic on Galaxy? It only detects one read instead of two. I followed ever...
• Help with Cuffdiff - metadata malues missing
  I am trying to run differential expression analysis using Cuffdiff in Galaxy. I have two conditio...
• Assembly of metatranscriptomic data with reference
  Hello. I have a set of reads produced by metatranscriptomic cDNA sequensing of the total RNA samp...
• hg38 GFF3/GTF source
  Hi, I am trying to use cuffdiff to compare relative gene expression from some human cell line sa...
• Interpreting detected "duplicates" between different tools
  Hello. When I analize my data in FASTQC, the SEQUENCE DUPLICATION LEVELS are ok (green lavel and ...
• analysis of tophat results
  hi i have done qc and then groomed single end sra sequences from ebi. i ran tophat. i am getting...
• Troubles with full settings on Lastz
  Hi, I am new to bioinformatics and I have troubles using Lastz. I use it to detect a 39nt seque...
• Trimmomatic data detection
  Hello I have a problem by detection data sequences uploaded in format fastqsanger by Tools/NGS :...
• Error running BWA-MEM or Bowtie2 in usegalaxy
  Hi, I am getting following errors while trying to run BWA-MEM or bowtie2 in usegalaxy. The input...
• An error occurred setting the metadata for this dataset
  Hi Galaxy Team, I've been using TopHat to map my reads and I've received the same error message ...
• Bowtie2/FreeBayes/mpileup variant detection on NGS of PCR amplicons around Cas9/CRISPR indels
  Hey threre, I have an MiSeq experiment using 24 indices where in each index I was sequencing 3 P...
• Using filtered data for TopHat
  Hi, When I extract DRR data using "Extract reads in FASTQ/A format from NCBI SRA," for TopHat, ...
• snp detection for sra files
  hello i need an urgent help i want to detect snp for sra files i did bwa and convert the file i...
• multi bam summary
  Hello, I was trying to use mutibamsummary tool to generate the read coverage for 8 filtered Bam ...
• TopHat splice junctions
  Dear all, I'm trying to detect alternative splicing events from RNA-seq experiments. I have used...
• Is it possible to auto-detect the database/genome of the input file?
  Hi. I am writing xml scripts that will ask the users to pick a reference genome (e.g. hg19, mm10,...
• chimeric transcripts detection tool
  Hi, How many tools can one find in the galaxy local too shed for detection of chimeric/fusion tr...
• Problem In Manage Data Library
  We have found that, in Galaxy, when we add dataset to a library under Manage data libraries, Gala...
• My Female subjects seem to be expressing Y Chromosome Genes
  Hi, I have recently sequenced extracted RNA from plasma samples taken from female subjects. Upon...
• Problems with MACS peak calling--FDRs of 100%
  Hello, I am having some issues with MACS peak calling. I have 5 files in my ChIP-Seq dataset (in...
• TopHat - set metadata manually or retry auto-detection
  "An error occurred setting the metadata for this dataset Set it manually or retry auto-detection"...
• Continuous processing for jobs where metadata is auto-detected
  I have a local installation of galaxy. I designated that my job "auto-detect metadata." The jo...