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Question: analysis of tophat results
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gravatar for 1603.neha
2.6 years ago by
1603.neha • 70
1603.neha • 70 wrote:

hi i have done qc and then groomed single end sra sequences from ebi. i ran tophat. i am getting error- An error occurred setting the metadata for this dataset Set it manually or retry auto-detection.

though the align summary result says.Reads: Input : 14720816 Mapped : 14253449 (96.8% of input) of these: 1397422 ( 9.8%) have multiple alignments (34022 have >20) 96.8% overall read mapping rate.

please help. thanks
tophat rnaseq • 1.0k views
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modified 2.6 years ago • written 2.6 years ago by 1603.neha • 70

accepted hits and align summary of tophat output i showing this error - An error occurred setting the metadata for this dataset Set it manually or retry auto-detection. for all the 6 sra files i am working on. thanks

ADD REPLY • link written 2.6 years ago by 1603.neha • 70
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gravatar for Jennifer Hillman Jackson
2.6 years ago by
Jennifer Hillman Jackson ♦ 25k
United States
Jennifer Hillman Jackson ♦ 25k wrote:

Hello,

These are probably server related problems that we now believe are cleared up. Please click on the pencil icon per dataset to reach the Edit Attributes form. The first tab will have a button to auto-detect metadata. Some datasets may have a direct link to do this within them (inside a yellow box at the top of the green dataset box in the history panel).

Allow that processing to complete before queuing up jobs that use the corrected datasets as input.

Thanks, Jen, Galaxy team
ADD COMMENT • link written 2.6 years ago by Jennifer Hillman Jackson ♦ 25k
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gravatar for 1603.neha
2.6 years ago by
1603.neha • 70
1603.neha • 70 wrote:

thanks a lot jen and galaxy team
ADD COMMENT • link written 2.6 years ago by 1603.neha • 70
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